ForteBio has launched the Dip and Read Anti-GST and the Amine Reactive Second-Generation (AR2G) biosensors for use on its Octet instrumentation platform.
ForteBio Dip and Read Anti-GST Biosensor
The Anti-GST biosensor allows researchers to perform kinetic and quantitative analyses of GST fusion proteins easily, cost effectively and with high-throughput. It allows kinetic characterization of macromolecular type interactions that occur between target analytes and GST-tagged proteins. It also allows quick and easy quantification of GST-tagged proteins in bacterial, media or buffer lysates. Using normal low-pH protocol, the biosensors can be regenerated 10 times.
The new Amine Reactive Second-Generation biosensor allows kinetic characterization of macromolecular type interactions that occur between target analytes and purified proteins. It provides high-throughput and is as robust as the company’s Streptavidin biosensor. The AR2G biosensor also allows an alternative biosensor surface that may be required for particular ligands.
The vice president of marketing at ForteBio, Christopher Silva, stated that the AR2G biosensor was an enhancement of the company’s first-generation AR biosensor and that it had more signal intensity during the immobilization of ligands and detection of analytes during assays. The AR2G also features higher stability, lower non-specific binding and higher loading density in various types of salts and pH conditions.
ForteBio has built the Octet platform based on its proprietary BioLayer Interferometry (BLI) technology that utilizes optical biosensors for measuring numerous interactions in parallel. The Octet platform features 8- and 16-channel instruments that have the capability to accommodate 96- and 384-well assay formats. The Octet biosensors can be configured in a typical microplate format. A proprietary biocompatible matrix that has uniformity, non-denaturing and minimal non-specific binding properties is coated onto the Octet biosensors.