Mar 13 2015
A victim of cyanide poisoning can die within 30 minutes.
The diagnostic test to determine cyanide exposure takes 24 hours.
Firefighters battle a Nov. 19, 2014, blaze at a grain elevator in Mitchell, South Dakota, from the outside to prevent exposure to chemicals. The building, which is owned by CHS Farmers Alliance, contained farm chemicals and fertilizer. Photo by Matthew Gade of the Mitchell Daily Republic
According to U.S. Department of Health recommendations, “any delay to draw blood or collect urine could endanger patient welfare.” That’s why medical personnel simply treat the patient when dealing with a possible case of cyanide poisoning.
Associate professor Brian Logue of the South Dakota State University chemistry and biochemistry department has a solution—a prototype that can detect exposure to cyanide within 70 seconds. Through funding from the Department of Defense and National Institutes of Health, Logue has been analyzing cyanide and its metabolites to help create countermeasures for cyanide exposure.
Firefighters, industrial workers at risk
When an industrial fire occurs, workers and firefighters can be exposed to cyanide, according to Logue.
Scientists and medical personal need a quick, reliable and portable means of identifying exposure to cyanide, Logue explained, which prompted the NIH’s request for proposals.
Early symptoms of cyanide poisoning include dizziness, headache, shortness of breath and rapid heart rate as well as nausea and vomiting, according to the Centers for Disease Control and Prevention.
“A lot of these symptoms are pretty nondescript,” Logue pointed out. “That’s another part of the reason for the sensor."
Quick diagnosis
The patent-pending sensor design uses a two-stage process—the first converts the cyanide in the sample into a gas and the second traps it in a base. “It diffuses like perfume,” Logue said.
The cyanide then reacts with a second substance and becomes fluorescent when exposed to light, according to postdoctoral researcher Randy Jackson. The fluorescent product containing cyanide then emits its own light which can be measured to determine analytical concentration.
Though diagnostic time has decreased from 2 minutes to 70 seconds, Logue pointed out, “we haven’t optimized it—we’d like that to be under a minute.”
In an emergency scenario, a blood sample from a finger prick, like a diabetic would do to measure blood sugar, could be used to determine if a person has been exposed to cyanide, Jackson explained.
Ultimately, the researchers would like to be able to test saliva, because typically cyanide exposure occurs through the mouth or nose, Logue explained. However, thus far a consistent link between the two has not been established.
Accuracy, design optimization
Tests performed on rabbits were 100 percent accurate at diagnosing exposure to cyanide, even at levels 200 times lower than the lethal amount, Logue explained. The device detects cyanide levels below what a person is exposed to when smoking a cigarette.
Logue plans on conducting larger animal studies to statistically determine sensor accuracy. “We’re pretty confident the device is accurate diagnostically because the specificity of the reaction is very good.”
In addition, the researchers will tap engineering expertise to miniaturize the device and will optimize the replaceable cartridge that contains the reactive chemicals. Eventually, the device must be approved by Federal Drug Administration.
Refining and getting this device to market is Logue’s mission: “I want to get this product to the people to help save lives.”
About South Dakota State University
Founded in 1881, South Dakota State University is the state’s Morrill Act land-grant institution as well as its largest, most comprehensive school of higher education. SDSU confers degrees from eight different colleges representing more than 175 majors, minors and specializations. The institution also offers 32 master’s degree programs, 15 Ph.D. and two professional programs. The work of the university is carried out on a residential campus in Brookings, at sites in Sioux Falls, Pierre and Rapid City, and through Cooperative Extension offices and Agricultural Experiment Station research sites across the state.